## Abstract The primary objective of this work was to create a cellβfree protein synthesis extract that produces proteins requiring disulfide bonds while using glucose as an energy source. We attempted to avoid using iodoacetamide (IAM) to stabilize the required oxidizing thiol redox potential, sin
Prolonged production of proteins in a cell-free protein synthesis system using polymeric carbohydrates as an energy source
β Scribed by Ho-Cheol Kim; Tae-Wan Kim; Dong-Myung Kim
- Book ID
- 108254840
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 459 KB
- Volume
- 46
- Category
- Article
- ISSN
- 1873-3298
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract The accumulation of inorganic phosphate inhibits protein synthesis in cellβfree protein synthesis reactions that are energized by highβenergyβphosphateβcontaining compounds. This study developed a new scheme for supplying energy using dual energy sources to enhance the regeneration of A
## Abstract Herein we describe the methods for selective and reversible regulation of gene expression using antisense oligodeoxynucleotides (ODNs) in a cellβfree protein synthesis system programmed with multiple DNAs. Either a complete shut down or controlled level of gene expression was attained t
Dihydrofolate reductase was synthesized in a batch system in the presence of the af6Nty ligand methotrexate, bound to various matrices. l k o types of gel were used: commercial methotrexate-agarose with pores inaccessible for translation machinery and methotrexate-POROS with pores easily accessible