Prolonged hepatitis C virus seroconversion in a blood donor, detected by HCV Antigen test in parallel with HCV RNA
โ Scribed by M. Alvarez; D. Planelles; E. Vila; J. Montoro; E. Franco
- Book ID
- 110744274
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 54 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0042-9007
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Approximately 90% of subjects with chronic hepatitis resulting from hepatitis C virus infection have hepatitis C virus RNA in serum. However, the prevalence of hepatitis C virus RNA in serum from subjects with hepatitis C virus antibody associated with persistent normal liver biochemical values is u
Serum samples from 103 blood donors or patients with slightly increased serum levels of liver enzymes were tested for antibodies to hepatitis C virus (anti-HCV) using second generation tests and for HCV RNA by the polymerase chain reaction (PCR). PCR was in a nested configuration, using primer pairs
## Abstract Hepatitis C virus (HCV) requires reverse transcriptaseโpolymerase chain reaction (RTโPCR) or branched DNA signal amplification assays to be detected in patient samples. Although conventional methods of RNA isolation are employed for samples of serum, plasma, and peripheral blood mononuc