Proliferative potential and K-ras mutation in epithelial hyperplasia of the gallbladder in patients with anomalous pancreaticobiliary ductal union
✍ Scribed by Satoshi Tanno; Takeshi Obara; Tsuneshi Fujii; Yusuke Mizukami; Ryushi Shudo; Noriyuki Nishino; Hitoshi Ura; Andres J. P. Klein-Szanto; Yutaka Kohgo
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 197 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0008-543X
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✦ Synopsis
Recent studies have shown that anomalous pancreaticobiliary ductal union (APBD) is an important risk factor for the development of gallbladder carcinoma. Epithelial hyperplasia of the gallbladder is one of the characteristic changes, but it is not clear whether epithelial hyperplasia is a premalignant lesion that could lead to cancer in APBD patients.
METHODS.
Twenty-four APBD patients were classified into two types: patients with bile duct dilation (dilated type) (n ϭ 13) and patients without dilation (undilated type) (n ϭ 11). Resected gallbladders obtained from APBD patients and control patients without APBD were examined histologically and with immunohistochemical techniques for the detection of p53 and Ki-67 (as a cell proliferation marker). K-ras mutations were examined using polymerase chain reaction-restriction fragment length polymorphism and direct DNA sequence analysis. The patients were also classified, according to extent of epithelial hyperplasia, as having high grade or low grade hyperplasia.