Proliferative activity in gastric cancer determined with cell cycle-related monoclonal antibodies Ki-67 and p105: Analysis by flow cytometry
✍ Scribed by Hironobu Kimura; Yutaka Yonemura; Itsuo Miyazaki
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 640 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0022-4790
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✦ Synopsis
The proliferative activity of gastric cancer cells was determined by DNA flow cytometric (FCM) analysis and labeling rates of Ki-67 and monoclonal antibodies and proliferation-associated nuclear antigen (p1OS) autoantibodies in 28 patients with fresh human gastric cancer cells. By setting the cutoff line at the level as used in a negative control study without primary antibody in the same sample, the Ki-67 and p105 labeling rates were calculated by the dual fluorescence analysis. A total of 43 experiments was performed on FCM analysis for each antigen: 28 with Ki-67 and 15 with p105. The mean Ki-67 labeling rate of gastric cancer cells was 45.1% (13.9-76.3%). The Ki-67 labeling rates were significantly higher for larger size tumor, peritoneal metastasis, and advanced clinical stage. A significant correlation was found between Ki-67 labeling rate and p105 labeling rate ( P < 0.0s). Bivariate FCM may be an easy method for obtaining useful information of cell kinetics.