Proliferation index and vascular density of giant cell tumors of bone: Are they prognostic markers?

✍ Scribed by Muhammadsamir A. Sulh; M. Alba Greco; Tony Jiang; Sunanda B. Goswami; David Present; German Steiner

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 804 KB
• Volume: 77
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/(sici)1097-0142(19960515)77:10<2044::aid-cncr12>3.0.co;2-v

✦ Synopsis

BACKGROUND.

The proliferation index (PI) and vascular density OD) in giant cell tumors (GCT) of bone were studied to assess their value as prognostic markers.

METHODS.

Formalin fixed, paraffin embedded tissue from 7 nonrecurrent (NR-GCT) and 13 recurrent (R-GCT) tumors were stained with MIB-1, a monoclonal antibody against Ki-67 (nuclear proliferation antigen), and QBEND-10, a monoclonal antibody against CD34 (endothelial antigen). A minimum of ten fieldsicase were analyzed on the SAMBA 4000 Image Analyzer. Only mononuclear cell nuclei were analyzed for MIB-1 staining.

RESULTS.

Mean values 2 1 Standard deviation (SD) for PI (stained nuclear area/ total nuclear area) were: NR-GCT, 10.98 2 3.70; R-GCT (initial presentation), 8.94 i 3.57; and R-GCT (first recurrence), 9.25 + 3.52. In addition, the mean PI 2 1 SD