✦ LIBER ✦

Proliferation and differentation of normal granulopoietic cells in continuous bone marrow cultures

✍ Scribed by Neil Williams; Heather Jackson; Enrique M. Rabellino

Publisher: John Wiley and Sons
Year: 1977
Tongue: English
Weight: 440 KB
Volume: 93
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040930315

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Modified conditions are reported for successful continuous bone marrow cultures with stem cell self‐renewal and granulocyte‐macrophage differentiation. Cells cultured over several weeks were found to be identical to freshly isolated bone marrow cells. Polymorphic neutrophils derived from cultures and primary bone marrow neutrophils both showed C~3~ and IgG receptors and both actively phagocytosed foreign particles. Cultured and normal CFU‐c were identical, both in their dose responsiveness to CFS and in their sedimentation rate characteristics.

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