Proliferating cell nuclear antigen and p53 expression as prognostic factors in T1-2MO prostatic adenocarcinoma

✍ Scribed by Satu L. B. Vesalainen; Pertti K. Lipponen; Martti T. Talja; Esko M. Alhava; Kari J. Syrjánen

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: French
• Weight: 784 KB
• Volume: 58
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910580226

✦ Synopsis

The expression of proliferating cell nuclear antigen and p53 protein was analysed by immunocy-tochemical methods (PC 10, CM I antisera) in I39 patients with TI -2M0 prostatic adenocarcinomas followed-up for > 12 years. p53 protein was expressed in 2 I ( IS0/o) tumours ( 15%). the fraction of positive nuclei being very low (mean SE, I% * 0.7%). Accumulation of p53 protein in epithelial cells was independent of tumour stage and Gleason score, and had no effect on prognosis. In 4 cases, p53 protein was expressed only in stromal cells. The fraction of PCNApositive nuclei (evaluable in I 16 cases) was higher in T2 than in TI tumours (p < 0.001); furthermore, high Gleason score was positively correlated with PCNA positivity (p < 0.001). A finding of over 5% of PCNA-positive nuclei predicted progression in T and M categories and were a sign of poor outcome. The fraction of PCNA-positive stromal-cell nuclei was related to T-category with a borderline significance (p = 0.06). In a multivariate analysis of the prognostic factors, independent predictors of survival included Gleason score (p < O.OOl), fraction of PCNA-positive nuclei (p = 0.01 3), observation before therapy (p = 0.05), and T-category (p = 0.07) in that order of significance. The results suggest that overexpression of p53 protein is of marginal prognostic value in local prostatic adenocarcinomas. whereas direct measurement of cell proliferation by PCNA immunolabelling provides important prognostic information in TI -2M0 tumours, in addition to the Gleason score.