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Prokaryotic expression and purification of Camellia sinensis polyphenol oxidase

✍ Scribed by Jing-wei Liu; You-yi Huang; Jian Ding; Cong Liu; Xiu-dan Xiao; De-jiang Ni

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
122 KB
Volume
90
Category
Article
ISSN
0022-5142

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## Abstract Polyphenol oxidase extracted from oil bean (__Pentaclethra macrophylla__) seeds was purified 165‐fold over the crude enzyme extract. The apparent molecular weight of the enzyme by gel filtration was 110.8 k Β± 9.0 k while SDS‐PAGE indicated a single species of molecular weight 28.0 k. A