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Proinsulin: Crystallization and Preliminary X-Ray Diffraction Studies

✍ Scribed by W. Wardle Fullerton, Reginald Potter and Barbara W. Low


Book ID
123649566
Publisher
National Academy of Sciences
Year
1970
Tongue
English
Weight
944 KB
Volume
66
Category
Article
ISSN
0027-8424

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Crystallization and preliminary x-ray di
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Human procathepsin L has been expressed in the yeast Pichiapastoris and its inactive (Cys25Ser) and unglycosylated (ThrllOAla) mutant purified, concentrated to 4 mglml, and crystallized by vapor diffusion against solution containing 1.4 M (Na,K)PO, buffer, pH 7.8. Crystal size was increased by multi