Progress and variations in two-hybrid and three-hybrid technologies

✍ Scribed by Becky L Drees

• Book ID: 104415193
• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 102 KB
• Volume: 3
• Category: Article
• ISSN: 1367-5931
• DOI: 10.1016/s1367-5931(99)80012-x

✦ Synopsis

The original yeast two-hybrid system and its variants have proven to be effective tools for identification and analysis of protein-protein, protein-DNA and protein-RNA interactions. The two-hybrid assay is being applied to the entire complement of proteins of the yeast Saccharomyces cerevisiae to characterize the network of protein-protein interactions in the eukaryotic cell. The development of nontranscriptional cytosolic and membrane-associated two-hybrid methods has made it possible to detect and examine a number of protein-protein interactions in their normal cellular locations. Small-molecule hybrid systems have been developed which can be used to study protein-ligand interactions and to activate cellular processes by forcing protein associations.