Programmed Necrosis: Methods and Protocols

✍ Scribed by Adrian T. Ting

• Publisher: Springer New York;Humana Press
• Year: 2018
• Tongue: English
• Leaves: 191
• Series: Methods in Molecular Biology 1857
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This volume explores the latest techniques and approaches used to study this emerging form of cell death. The chapters in this book cover topics such as distinguishing necroptosis from apoptosis; detecting MLKL oligomerization during programmed necrosis; analyzing the RIPK3 necrosome complex; using RIPK1 kinase small molecule inhibitors in studying necroptosis; monitoring RIPK1 phosphorylation in the TNFR1 signaling complex; and assessing in vivo kidney cell death in glomerular injury. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Programmed Necrosis: Methods and Protocols is a valuable resource for technicians, graduate students, postdoctoral fellows, and other scientists who are pursuing studies in this field.

✦ Table of Contents

Front Matter ....Pages i-xi
Tools in the Art of Studying Necroptosis (Adrian T. Ting)....Pages 1-9
Loss-of-Function RNAi Screen to Identify Necrosis-Signaling Molecules (David Mark Moquin, Francis Ka-Ming Chan)....Pages 11-18
Chemical Library Screens to Identify Pharmacological Modulators of Necroptosis (Danish Saleh, Alexei Degterev)....Pages 19-33
Distinguishing Necroptosis from Apoptosis (Inbar Shlomovitz, Sefi Zargarian, Ziv Erlich, Liat Edry-Botzer, Motti Gerlic)....Pages 35-51
Methods for Studying TNF-Mediated Necroptosis in Cultured Cells (Zikou Liu, John Silke, Joanne M. Hildebrand)....Pages 53-61
Analysis of Necroptosis in Bone Marrow-Derived Macrophages (Diana Legarda, Adrian T. Ting)....Pages 63-70
Generation and Use of Chimeric RIP Kinase Molecules to Study Necroptosis (Diego A. Rodriguez, Douglas R. Green)....Pages 71-83
Detection of MLKL Oligomerization During Programmed Necrosis (Zhenyu Cai, Zheng-Gang Liu)....Pages 85-92
Analysis of Cytokine- and Influenza A Virus-Driven RIPK3 Necrosome Formation (Roshan J. Thapa, Shoko Nogusa, Siddharth Balachandran)....Pages 93-99
Detection of RIPK1 in the FADD-Containing Death Inducing Signaling Complex (DISC) During Necroptosis (Rosalind L. Ang, Adrian T. Ting)....Pages 101-107
Use of RIP1 Kinase Small-Molecule Inhibitors in Studying Necroptosis (Allison M. Beal, John Bertin, Michael A. Reilly)....Pages 109-124
Analyzing Necroptosis Using an RIPK1 Kinase Inactive Mouse Model of TNF Shock (Matija Zelic, Michelle A. Kelliher)....Pages 125-134
Assessment of In Vivo Kidney Cell Death: Acute Kidney Injury (Wulf Tonnus, Moath Al-Mekhlafi, Christian Hugo, Andreas Linkermann)....Pages 135-144
Assessment of In Vivo Kidney Cell Death: Glomerular Injury (Wulf Tonnus, Moath Al-Mekhlafi, Florian Gembardt, Christian Hugo, Andreas Linkermann)....Pages 145-151
Detection of Necroptosis by Phospho-RIPK3 Immunohistochemical Labeling (Joshua D. Webster, Margaret Solon, Susan Haller, Kim Newton)....Pages 153-160
Characterization of the TNFR1-SC Using “Modified Tandem Affinity Purification” in Conjunction with Liquid Chromatography–Mass Spectrometry (LC-MS) (Matthias Reichert, Amandeep Bhamra, Sebastian Kupka, Henning Walczak)....Pages 161-169
Monitoring RIPK1 Phosphorylation in the TNFR1 Signaling Complex (Dario Priem, Yves Dondelinger, Mathieu J. M. Bertrand)....Pages 171-179
Analysis of CYLD Proteolysis by CASPASE 8 in Bone Marrow-Derived Macrophages (Diana Legarda, Adrian T. Ting)....Pages 181-188
Back Matter ....Pages 189-191

✦ Subjects

Life Sciences; Cell Biology