Prognostic relevance of MIB-1 immunoreactivity, S-phase fraction, 5-bromo-2′-deoxyuridine labeling indices, and mitotic figures in gliomas
✍ Scribed by H. Struikmans; D.H. Rutgers; G.H. Jansen; H.F.J. Dullens; L. Oosten; C.A.F. Tulleken; I. van der Tweel; J.J. Battermann
- Book ID
- 101302694
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 127 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1065-7541
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✦ Synopsis
Prognostic relevance of cell proliferation markers was evaluated in 27 glioma patients. By 1) flow cytometry (FCM), i.e., S-phase fraction (SPF), and BrdUrdlabeling index (LI fcm ); 2) immunohistochemistry (IHC), i.e., BrdUrd-labeling index (LI ihc ) and MIB-1 immunoreactivity (MIB-1 LI ihc ); and 3) histologic examination, i.e., the presence or absence of cells in mitoses, were assessed. A longer local progression free survival (LPFS) was significantly associated with low SPF, low LI fcm , and low MIB-1 LI ihc . For LI ihc , no significant association was found. LI fcm appeared to be a more promising prognosticator than MIB-1 LI ihc . In comparison with this marker, the presence or absence of mitotic figures appeared to be an even stronger prognosticator. Prognostic significance of LI fcm appeared to be of importance in low-grade gliomas. The number of patients in our study is limited. Our findings were: 1) the presence or absence of cells in mitoses (M-phase activity) appeared to be of more prognostic significance than LI fcm (S-phase activity) and MIB-1 LI ihc (non-G0-phase activity); 2) of the tested experimental cell proliferation markers, LI fcm appeared to be of more prognostic significance than MIB-1 LI ihc , SPF, and LI ihc ; and 3) LI fcm is likely to be an important prognosticator in low-grade gliomas and is, therefore, not definitive and only of potential interest.