✦ LIBER ✦

PROfusion™: Genetically Tagged Proteins for Functional Proteomics and Beyond

✍ Scribed by Brent L. Kreider

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 39 KB
Volume: 20
Category: Article
ISSN: 0198-6325
DOI: 10.1002/(sici)1098-1128(200005)20:3<212::aid-med5>3.0.co;2-f

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✦ Synopsis

As a new enabling technology in functional genomics, Phylos, Inc. has pioneered PRO-fusion™ technology, whereby proteins are covalently tagged with their own genetic information (mRNA). Using this technology, both synthetic and natural libraries (representing the repertoire of proteins naturally expressed in a given cell type or tissue source) have been constructed. Due to the in vitro nature of library construction, Phylos libraries are the largest described to date, up to 10 14 in size. From a library of such molecules, one can select for a protein function of choice with the benefit that the genetic material is linked to the protein for subsequent PCR amplification, enrichment, and, ultimately, identification.

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