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Profiling of membrane proteins from human macrophages: Comparison of two approaches

✍ Scribed by Marie-Christine Slomianny; Annabelle Dupont; Fatiha Bouanou; Olivia Beseme; Anne-Laure Guihot; Philippe Amouyel; Jean-Claude Michalski; Florence Pinet


Book ID
118285690
Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
707 KB
Volume
6
Category
Article
ISSN
1615-9853

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Two-dimensional electrophoresis of proteins is often precluded due to the lack of solubilization of cell membrane extracts in an aqueous medium. Various additives and detergents have been used to circumvent the problem, but their efficacy may not be satisfactory. In this study, the removal of lipidi