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Profiling of 2-aminoacridone derivatised glycans by electrospray ionization mass spectrometry

✍ Scribed by Joanne Charlwood; Jim Langridge; David Tolson; Helen Birrell; Patrick Camilleri

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
101 KB
Volume
13
Category
Article
ISSN
0951-4198

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✦ Synopsis

Three different types of N-glycans, high-mannose, complex and hybrid, have been derivatised with 2aminoacridone and analysed by electrospray mass spectrometry and tandem mass spectrometry (MS/MS) using a hybrid quadrupole orthogonal acceleration time-of-flight mass spectrometer, fitted with a nanoflow electrospray ion source. Relatively simple MS/MS fragmentation patterns have been observed for each type of glycan, allowing rapid elucidation of the order in which the monosaccharide residues making up these glycans are linked to one another.

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