𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Profiling Kinase Activities by Using a Peptide Chip and Mass Spectrometry

✍ Scribed by Dal-Hee Min; Jing Su; Milan Mrksich

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
210 KB
Volume
116
Category
Article
ISSN
0044-8249

No coin nor oath required. For personal study only.

✦ Synopsis

Herein we describe a strategy that combines peptide chips with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) to evaluate kinase activities rapidly and semi-quantitatively. We have already shown that monolayers of alkanethiolates on gold are wellsuited as substrates for MALDI-TOF MS, and could be used to measure enzyme activities and to perform high-throughput screenings. [1,2] A significant aspect of this approach-which we term SAMDI (self-assembled monolayers for MALDI) MS-is that it avoids the use of labels, greatly simplifying the formatting of assays. Herein we establish two important

πŸ“œ SIMILAR VOLUMES

Profiling Peptide Adducts of Oxidized N-
Profiling Peptide Adducts of Oxidized N-Acetyldopamine by Electrospray Mass Spectrometry
✍ James L. Kerwin πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 197 KB πŸ‘ 1 views

Phenolic and catecholamine polymers are common constituents of many biological systems. Cross-linking of polyphenols with other phenols, peptides, proteins and carbohydrates results in the synthesis of complex natural products which are not easily characterized. Electrospray mass spectrometry (ES-MS

Identification of by-products from an or
Identification of by-products from an orthogonal peptide ligation by oxime bonds using mass spectrometry and tandem mass spectrometry
✍ Corinne BurΓ©; Dominique LeliΓ¨vre; AgnΓ¨s Delmas πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 111 KB πŸ‘ 1 views

Synthetic proteins with unusual architecture are obtained through chemoselective ligation, a method based on the condensation of unprotected peptides under mild aqueous conditions. The last step of a new procedure leading to a tri-branched conjugate consists of the chemoselective ligation reaction b

Analysis of a Standard Peptide Mixture b
Analysis of a Standard Peptide Mixture by Capillary Electrophoresis with Mass Spectrometry and with Tandem Mass Spectrometry
✍ H. J. Major; A. E. Ashcroft πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 409 KB πŸ‘ 2 views

The combination of capillary electrophoresis with mass spectrometry allows efficient separation and identification of components in mixtures with greater specificity than can be obtained using capillary electrophoresis and ultra violet detection alone. For the mixture of peptides analysed in this ap