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Profile and Flight Time Analysis of Bovine Insulin Clusters as a Probe of Matrix-assisted Laser Desorption/Ionization Ion Formation Dynamics

✍ Scribed by Kinsel, Gary R.; Edmondson, Ricky D.; Russell, David H.

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
361 KB
Volume
32
Category
Article
ISSN
1076-5174

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✦ Synopsis

Detailed ion signal proÐle and Γ‘ight time analyses were performed on the time-of-Γ‘ight signals obtained for bovine insulin cluster ions produced by matrix-assisted laser desorption/ionization (MALDI). ProÐle analyses of the ion signals strongly suggest that the signals are made up of a composite of two separate components and analysis of the Γ‘ight times of the two components suggests that the ions are formed with di †erent dynamics. The formation dynamics of one component of the ions is best described as prompt ionization to yield ions with essentially massindependent total energies. The formation dynamics of the second component of the ions is best described as delayed gas-phase ionization of entrained material moving at a constant velocity. This model is supported by several ancillary experiments and suggests new insights into the mechanism of MALDI ion formation.

by John ( Wiley & Sons, Ltd.

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