Products of denitrification by a marine bacterium as revealed by gas chromatography

✍ Scribed by J. M. Barbaree; W. J. Payne

• Book ID: 104750127
• Publisher: Springer-Verlag
• Year: 1967
• Tongue: English
• Weight: 465 KB
• Volume: 1
• Category: Article
• ISSN: 0025-3162
• DOI: 10.1007/bf00386519

✦ Synopsis

Gas chromato~aphy was used to analyse the gases released by growing cultures of the denitrifying marine bacterium Pseudomonas perfectomarinus. When nitrate was provided as final oxidant, CO 2 was the first detectable gas released. Following the peak rate of release of C02 at approximately 16 h, N~ liberation began and peaked at 30 h. When N20 was provided as terminal oxidant, CQ and N~ reIease began within a few hours and the rate of liberation of both increased for 45 to 50 h before leveling. Cell-free extracts of bacteria from denitrifying cultures of P. ~erfectomarinus were incubated anaerobically with nitrate, malie acid and electron transfer cofaetors provided, and the gases in the atmosphere above the reaction mixtures were analysed. NO was found to be present transiently, and N20 was discerned after prolonged incubation. The applicability of gas ellromatography to analyses of marine ecosystems and the need for better means of sampling the atmosphere above enzymatic reaction mixtures were discussed.