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Production, purification and characterization of a thermostable laccase from a tropical white-rot fungus

✍ Scribed by Li-Qiong Guo; Shuo-Xin Lin; Xiao-Bing Zheng; Zi-Rou Huang; Jun-Fang Lin

Publisher: Springer
Year: 2010
Tongue: English
Weight: 243 KB
Volume: 27
Category: Article
ISSN: 1573-0972
DOI: 10.1007/s11274-010-0502-8

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## Abstract The wood‐degrading fungus __Pleurotus__ __pulmonarius__ produces at least two laccase isoforms, Lcc1 and Lcc2, when grown on wheat bran solid state medium. The main laccase, Lcc2, was purified to apparent electrophoretic homogeneity by using acetone precipitation, anion‐exchange chromat