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Production of virus resistant and insect tolerant transgenic tobacco plants

✍ Scribed by Xiao-you Liang; Yu-xian Zhu; Jing-jiu Mi; Zhang-liang Chen

Publisher
Springer
Year
1994
Tongue
English
Weight
876 KB
Volume
14-14
Category
Article
ISSN
0721-7714

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✦ Synopsis

The cucumber mosaic virus coat protein (CMV-CP) gene and a modified Bacillus thuringiensis Ξ΄-endotoxin (Bt toxin) gene were cloned into plant expression vector pE3. Tobacco (Nicotiana tabacum cv. G28) leaf discs were transformed with Agrobacterium tumefaciens A12 carrying recombinant pE14. Transgenic r0 and R1 tobacco plants expressing CMV-CP and Bt toxin genes were protected from CMV infection as well as feeding damage of Manduca Sexta (tobacco hornworm) larvae. These results demonstrate that it is feasible to breed new cultivars with multiple resistances via genetic engineering.

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