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Production of toluenecis-glycol by a recombinantEscherichia colistrain in a two-liquid phase culture system

✍ Scribed by J. T. Tsai; L. P. Wahbi; G. A. Dervakos; G. M. Stephens

Publisher
Springer Netherlands
Year
1996
Tongue
English
Weight
195 KB
Volume
18
Category
Article
ISSN
0141-5492

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✦ Synopsis

The recombinant Escherichia coli strain, TG2 (pi/l), can be used for biotransformation of toluene to toluene cis-glycol. However, toluene caused substantial inhibition of both growth and toluene cis-glycol production at concentrations above 0.04%. Tetradecane was found to be non-toxic at a phase ratio of 0.23 and provided protection against toluene toxicity. Thus, growth was still possible in the presence of 1.5% toluene. Furthermore, the yield of toluene ci.s-glycol was increased 1.7-fold compared with the yield in the absence of tetradecane.

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