Production of thermally induced recombinant proteins relative to cell biomass is influenced by cell density inEscherichia colibatch cultures
✍ Scribed by José Luis Corchero; Pau Vila; Rafael Cubarsi; Antonio Villaverde
- Book ID
- 104635266
- Publisher
- Springer Netherlands
- Year
- 1994
- Tongue
- English
- Weight
- 438 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0141-5492
No coin nor oath required. For personal study only.
✦ Synopsis
SUMMAR.Y Fed-batch cultures of recombinant Escherichiu coli were studied regarding their ability to permit CI857'"-controlled gene expression at different growth phases. Cells, growing at 50% p0, and 28°C in a bioreactor, were transferred to a shaker flask at 42°C and the product yield was analysed after a short incubation time. Within the batch period, the synthesis of recombinant 8galactosidase relative to the increase of biomass during the induction period was enhanced in an biomass-dependent fashion. Whereas in young cells biomass production dominates over recombinant protein production, the available energetic resources of aged cells seem to be employed more efficiently for CI857controlled biosynthesis of recombinant proteins than of cell material. A model to describe this 'altruistic' behaviour of old cultures is here proposed.