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Production of the metastatic phenotype by DNA transfection in a rat mammary model.

✍ Scribed by Barry R. Davies; Roger Barraclough; Michael P.A. Davies; Philip S. Rudland

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
587 KB
Volume
17
Category
Article
ISSN
1065-6995

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✦ Synopsis

Abstract

A syngeneic, immunocompetent rat mammary model has been employed to investigate the molecular basis of the metastatic phenotype. Transfection of the benign rat mammary epithelial cell line Rama 37 with the gene for p9Ka; a small, rat, calcium‐binding protein or DNA from metastatic human cell lines derived from a primary breast carcinoma or a pleural effusion yields transfectants with metastatic capabilities. Transfection of DNA from a benign human mammary cell line, a plasmid containing a cDNA for the human basic fibroblast growth factor (bFGF) gene, or the oncogene EJ‐ras‐1, fails to yield any transfectants expressing the metastatic phenotype.

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