Production of T-cell lines with inhibitory or stimulatory activity against syngeneic tumors in vivo. A preliminary report
✍ Scribed by J. J. Mulé; J. W. Forstrom; E. George; I. Hellström; K. E. Hellström
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- French
- Weight
- 429 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Abstract
We obtained Thy‐1‐positive cells directly from growing methylcholanthrene‐induced (MCA‐1510) sarcomas using fluorescence‐activated cell sorting, then cultured these lymphocytes in medium containing Interleukin‐2 and tested their activity in vivo against various MCA‐sarcoma lines with the Winn assay. We found that cultured T cells from small MCA‐1510 tumors (17 days after transplantation) significantly inhibited the growth of that particular sarcoma, but not of three other MCA‐tumor lines tested, while cultured T cells from large MCA‐1510 sarcomas (41 days after transplantation) significantly enhanced the growth of that tumor, but not of an unrelated tumor, MCA‐1460. The former cells were primarily Lyt‐1^+^, 2^+^ while the latter were primarily Lyt‐1^−^, 2^+^.