Production of subgroup-specific monoclonal antibodies against human rotaviruses and their application to an enzyme-linked immunosorbent assay for subgroup determination
✍ Scribed by Koki Taniguchi; Tomoko Urasawa; Shozo Urasawa; Toshio Yasuhara
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 688 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0146-6615
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✦ Synopsis
Nonneutralizing monoclonal antibodies were prepared against two strains, S2 and YO, of human rotaviruses isolated in cell culture. S2-37 and YO-5 antibodies had subgroup I and subgroup II specificities, respectively. The remaining antibodies (S2-65, YO-71, YO-89, and YO-156) reacted commonly with all the rotaviruses examined. All of the monoclonal antibodies agglutinated exclusively single-shelled particles and immunoprecipitated 42,000-dalton protein, a major component of inner capsid. Using the three monoclonal antibodies (S2-37, YO-5, and YO-156), an enzyme-linked immunosorbent assay was developed for detecting and subgrouping human rotavirus isolates.