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Production of Single-Stranded DNA Using a Uracil-N-glycosylase-Mediated Asymmetric Polymerase Chain Reaction Method

✍ Scribed by Jonathan K. Ball; Rebecca Curran


Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
196 KB
Volume
253
Category
Article
ISSN
0003-2697

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An optimized reaction condition for amplification of influenza A virus RNA, by thermus thermophilus (Tth) DNA polymerase-based PCR, incorporating uracil N glycosylase (UNG) and dUTP in the reaction has been determined. dUTP could not be substituted for all dTTP sites when UNG was present in the reac