Optimized PCR amplification of influenza
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Saibal K. Poddar; Mark H. Sawyer; James D. Connor
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Article
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1997
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John Wiley and Sons
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English
β 77 KB
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An optimized reaction condition for amplification of influenza A virus RNA, by thermus thermophilus (Tth) DNA polymerase-based PCR, incorporating uracil N glycosylase (UNG) and dUTP in the reaction has been determined. dUTP could not be substituted for all dTTP sites when UNG was present in the reac