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Production of salicylate hydroxylase from Pseudomonas putida UUC-1 and its application in the construction of a biosensor

✍ Scribed by Dao-Min Zhou; Poonam Nigam; John Jones; Roger Marchant

Publisher
Wiley (John Wiley & Sons)
Year
1995
Tongue
English
Weight
673 KB
Volume
64
Category
Article
ISSN
0268-2575

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✦ Synopsis

The enzyme salicylate hydroxylase was produced from Pseudomonas putida UUC-1 in an 80 dm3 16 h batch fermentation process yielding, -9 K units per 60 dm3. This enzyme preparation has been studied for its application in the construction of biosensor systems, e.g. carbon paste electrodes, screen-printed carbon electrodes, and disposable carbon enzyme electrodes, which will be used for the rapid estimation of salicylate in blood samples. The linear range of the disposable carbon enzyme electrode in response to salicylate was achieved to 1.8 mmol dm-3.

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