Production of recombinant endostatin from stably transformedTrichoplusia niBTI Tn 5B1-4 cells
β Scribed by JongMin Lee; KyungHwa Chang; JongHwa Park; YounHyung Lee; InSik Chung
- Book ID
- 110323521
- Publisher
- Springer Netherlands
- Year
- 2001
- Tongue
- English
- Weight
- 123 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0141-5492
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The effect of Tn 5B1-4 cell density at infection on the production of human secreted alkaline phosphatase (SEAP) was investigated using a split-flow air-lift bioreactor. Volumetric productivities of SEAP were highest when Tn 5B1-4 cells were infected at about 3 x 105 cells/cm2. Cell-to-cell contact
## Sulfated polyanions have been successfully significant advantage of this system is the high expresused to rapidly obtain and maintain stable single-cell sussion levels which can be achieved much more quickly pension of BTI-TN5B1-4 cells, a cell line which has a high than mammalian cell lines wh
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