𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Production of rabbit antibodies against the viral capsid antigen (VCA) of the epstein-barr virus (EBV)

✍ Scribed by B. F. Vestergaard; J. Hesse; B. Norrild; G. Klein

Publisher
John Wiley and Sons
Year
1978
Tongue
French
Weight
552 KB
Volume
21
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Rabbits were immunized with nuclear or cyto‐plasmic extracts of the Epstein‐Barr virus (EBV)‐producing marmoset cell line B95‐8. Following extensive absorption with human EBV‐negative cells (Hep‐2, Ramos and BJAB), sera were obtained that no longer reacted with cellular or serum proteins of human origin, but gave a single precipitin band with extracts of the human EBV‐producing line, P3HR‐1. Immunofluorescence tests performed with appropriate parallel human serum controls showed that the rabbit serum contained no activity against EBNA + EA ‐ VCA ‐ Raji cells, or against P3HR‐1 virus superinfected, cytosine arabinoside‐treated Raji cells that contained EBNA and EA, but not VCA. The sera gave a brilliant indirect immunofluor‐escence reaction with the virus‐producing (EBNA+EA+ VCA+) P3HR‐1 lines. Two‐color fluorescence tests, performed with a direct TRITC‐labelled VCA conjugate and indirect FITC‐staining with the rabbit serum, showed that the same cells reacted in both red and green fluorescence, confirming that the rabbit serum was directed specifically against some antigen formed in the virus‐producer cells. Since the synthesis of the relevant antigen was prevented by cytosine arabinoside it cannot be EA and must be a late antigen. The morphology and localization of the antigen support the conclusion that the antigen is VCA or some part of the VCA complex.

📜 SIMILAR VOLUMES

Column separation of viral capsid antige
Column separation of viral capsid antigen (vca)-positive cells from vca-negative cells in an epstein-barr virus (ebv)-producing lymphoid line
✍ Tina Dalianis; George Klein; Birger Andersson 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 French ⚖ 642 KB

## Abstract Virus producing, VCA (viral capsid antigen)‐positive cells could be selectively removed from Epstein‐Barr virus (EBV)‐carrying, virus‐producer P3HR‐1 cultures by two different methods of column passage. In the first, the virus‐producing cells were covered with human EBV antibody and sub

Avidities of IgG directed against viral
Avidities of IgG directed against viral capsid antigen or early antigen: Useful markers for significant epstein-barr virus serology
✍ Annika Andersson; Veronika Vetter; Ludger Kreutzer; Georg Bauer 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 729 KB

## Abstract Classical Epstein‐Barr virus (EBV) serology can be misleading in some cases due to the variability of the viral capsid antigen (VCA)‐IgM response, persistent or reactivated VCA‐IgM, or loss of anti‐EBNA‐1 during suppression of the cellular immune system. Therefore, we studied the useful

Expression of viral capsid protein antig
Expression of viral capsid protein antigen against Epstein-Barr virus in plastids of Nicotiana tabacum cv. SR1
✍ Maggie Y.T. Lee; Yuanxiang Zhou; Raymond W.M. Lung; Mee-Len Chye; Wing-Kin Yip; 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 279 KB

## Abstract Epstein‐Barr virus (EBV) infects nearly 90% of adults worldwide and is the pathogenic source of a broad spectrum of malignancies originating from lymphoid and epithelial cells. Currently, no vaccine has been developed to immunologically inactivate this virus. In infected patients, anti‐

Comparison of plasma Epstein–Barr virus
Comparison of plasma Epstein–Barr virus (EBV) DNA levels and serum EBV immunoglobulin A/virus capsid antigen antibody titers in patients with nasopharyngeal carcinoma
✍ Jian-Yong Shao; Yu-Hong Li; Hong-Yi Gao; Qiu-Liang Wu; Nian-Ji Cui; Li Zhang; Ga 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 213 KB 👁 2 views

## Abstract ## BACKGROUND Serologic measurement of antibodies to Epstein–Barr virus (EBV) immunoglobulin A/viral capsid antigen (IgA/VCA) and early antigen (IgA/EA) has been used widely to screen for nasopharyngeal carcinoma (NPC) in China. Recently, it was found that plasma EBV DNA concentration

Significance of Specific Epstein-Barr Vi
Significance of Specific Epstein-Barr Virus IgA and Elevated IgG Antibodies to Viral Capsid Antigens in Nasopharyngeal Carcinoma Patients
✍ Tuvia Hadar; Jack Sidi; Menashe Rahima; Erika Rakowsky; Ernesto Kahan; Batya Sar 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 611 KB

The feasibility of using elevated Epstein-Barr virus (EBV) specific-IgG antiviral capsid antigen (VCA) and IgA anti-VCA antibody levels as an aid in diagnosis of nasopharyngeal carcinoma (NPC) was analyzed by determination of serum antibody titers to EBV in 54 NPC patients, 114 healthy blood donors,

Increased incidence of IgA antibodies to
Increased incidence of IgA antibodies to the epstein-barr virus-associated viral capsid antigen and early antigens in patients with chronic lymphocytic leukemia
✍ Gottfried Döucen; Klaus J. Bross; Thomas Hecht; Wolfram Brugger; Georg W. Löhr; 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 French ⚖ 589 KB

## Freiburg, Fed. Rep. G e m n y . Antibody titers to Epstein-Barr virus (EBV)-associated early antigens (EA) and the viral capsid antigen (VCA) were determined by ELISA on 263 sera obtained from healthy donors, patients with Hodgkin's disease (HD), non-Hodgkin lymphomas (NHL), infectious mononucl