✦ LIBER ✦

Production of prostacyclin and fibrinolysis modulators by endothelial cells cultured in the presence of polyethylene terephthalate

✍ Scribed by Cenni, Elisabetta ;Ciapetti, Gabriela ;Cavedagna, Daniela ;Di Leo, Alessandro ;Pizzoferrato, Arturo

Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 373 KB
Volume: 27
Category: Article
ISSN: 0021-9304
DOI: 10.1002/jbm.820270906

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The aim of this study was the evaluation of the in vitro production of prostacyclin, and of tissue plasminogen activator (tPA) and its inhibitor, PAI‐1, by human endothelial cells cultured in the presence of polyethylene terephthalate (PET). After a 48 h contact between the cells and the polymer, the concentration of 6‐keto‐PGF~1α~, a stable metabolite of prostacyclin, tPA, and PAI‐1, was assayed on the supernatants. Contact of the endothelial cells with PET produced a highly significant reduction of 6‐keto‐PGF~1α~ with respect to control cultures. Tissue plasminogen activator concentration in the supernatants of the cultures in contact with the material was similar to that observed in the controls, while PAI‐1 production was significantly reduced. It can be concluded that the contact between endothelial cells and PET determines a reduction in the platelet aggregability and an increase of the fibrinolytic activity due to a decrease in PAI‐1, while tPA concentration remains unchanged. © 1993 John Wiley & Sons, Inc.

📜 SIMILAR VOLUMES

Stimulation of cell growth and inhibitio

Stimulation of cell growth and inhibition of prostacyclin production by heparin in human umbilical vein endothelial cells

✍ N. Hasegawa; M. Yamamoto; K. Yamamoto 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 535 KB

We characterized human umbilical vein (HUV) endothelial cells as to cell growth and prostacyclin production to get a better understanding of the properties of endothelial cells. Endothelial cell growth supplement (ECGS) and basic fibroblast growth factor (FGF) stimulated HUV endothelial cell growth.

The fibrinogen anion-binding exosite of

The fibrinogen anion-binding exosite of thrombin is necessary for induction of rises in intracellular calcium and prostacyclin production in endothelial cells

✍ Justinian R. Ngaiza; Geoffrey Manley; Jürgen Grulich-Henn; John L. Krstenansky; 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 719 KB

Thrombin stimulation of prostacyclin (PGI,) synthesis by cultured human umbilical vein endothelial cells (HUVEC) requires the active site of thrombin and involves rapid and transient rises in cytoplasmic free calcium [CaL+],. In this study, we investigated whether or not the anion-binding exosite fo

Induction of peroxisomal enzymes in cult

Induction of peroxisomal enzymes in cultured porcine endothelial cells by the hypolipidemic drug ciprofibrate

✍ Glauert, Howard P. ;Hennig, Bernhard ;Chow, Hannah S. 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 416 KB

## Abstract The purpose of this study was to determine if the hypolipidemic peroxisome proliferator ciprofibrate, which induces peroxisomes in the liver, can induce peroxisomes in cultured porcine pulmonary endothelial cells. Ciprofibrate was added at three concentrations to cell cultures for a 6‐d

Formation of depurinating N3Adenine and

Formation of depurinating N3Adenine and N7Guanine adducts by MCF-10F cells cultured in the presence of 4-hydroxyestradiol

✍ Muhammad Saeed; Eleanor Rogan; Sandra V. Fernandez; Fathima Sheriff; Jose Russo; 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 French ⚖ 200 KB

## Abstract Metabolic conversion of endogenous estrogens, estradiol (E~2~) and estrone (E~1~), to the catechol estrogens 4‐hydroxyE~1~(E~2~) [4‐OHE~1~(E~2~)] has been implicated in the initiation of cancer in rodents and humans. Evidence collected in our laboratories has shown that 4‐OHE~1~(E~2~) a

Modulation of bone ingrowth and tissue d

Modulation of bone ingrowth and tissue differentiation by local infusion of interleukin-10 in the presence of ultra-high molecular weight polyethylene (UHMWPE) wear particles

✍ Goodman, Stuart ;Trindade, Michael ;Ma, Ting ;Lee, Mel ;Wang, Neal ;Ikenou, Taka 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 201 KB

## Abstract Interleukin‐10 (IL‐10) is a cytokine that plays a major role in suppressing the inflammatory response, particulary cell‐mediated immunity that is characteristic of the TH1 response. The purpose of this study was to determine whether local infusion of IL‐10 could mitigate the suppression

High and low affinity binding sites for

High and low affinity binding sites for basic fibroblast growth factor on cultured cells: Absence of a role for low affinity binding in the stimulation of plasminogen activator production by bovine capillary endothelial cells

✍ David Moscatelli 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 834 KB

Scatchard analysis of binding of 125I-basic fibroblast growth factor (FGF) to baby hamster kidney (BHK) cells revealed the presence of two binding sites: a high affinity site with KD of 20 pM and 80,000 sites per cell and a low affinity site with KD of about 2 nM and 600,000 sites per cell. The bind