Production of poly-β-hydroxybutyrate byAlcaligenes eutrophustransformants harbouring clonedphbCAB genes

✍ Scribed by Jin-Seo Park; Hae-Chul Park; Tae-Lin Huh; Yong-Hyun Lee

• Book ID: 104633942
• Publisher: Springer Netherlands
• Year: 1995
• Tongue: English
• Weight: 356 KB
• Volume: 17
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00130360

✦ Synopsis

Three transformants of Afcufigenes eufrophus harbouring the recombinant plasmids containing phbCAE3, phbAE3, and phbC genes, were cultivated to investigate the effect of cloned genes on cell growth and poly-B-hydroxybutyrate accumulation. Both in the nutrient-rich and minimal media, the increased PUB accumulation in the transformants was observed compared to the parent strain, and this was the result of the increased enzyme activities in the transformants. Low carbon concentration and high C/N molar ratio favored higher PHI3 accumulations in the transformants. The transformant harbouring the phbC gene showed the highest PHB accumulation, which indicated that PHI3 synthase was the most critical enzyme for PHB biosynthesis in the transformant.