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Production of platelet-derived growth factor by cultured Wilms' tumor cells and fetal kidney cells

✍ Scribed by Dr. Gail E. Fraizer; Daniel F. Bowen-Pope; Dr. Arthur M. Vogel

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
694 KB
Volume
133
Category
Article
ISSN
0021-9541

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✦ Synopsis

We have cultured human Wilms' tumors (nephroblastomas) in vitro and examined their growth properties, tumorigenicity in nude mice, karyotypes, and production of a platelet-derived growth factor (PDGF)-like activity (PDGF,). The cultured Wilms' tumor cells do not exhibit transformed growth properties, senesce after 15-25 passages, and are not tumorigenic in nude mice. Two of t h e three Wilms' tumors examined had karyotypic abnormalities but none contained a deleted I l p region. The Wilms' tumor cells and control human fetal kidney cells produce significant amounts of PDGF, and express few PDGF receptors on the cell surface. Analysis of Wilms' tumor cell RNA using probes specific for PDGF A-chain and B-chain (c-sis) detects A-chain but not B-chain transcripts in the tumor cells.

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