Production of new CMS Brassica oleracea by transfer of `Anand' cytoplasm from B. rapa through protoplast fusion

The transfer of ‘Polima’ cytoplasmic mal

The transfer of ‘Polima’ cytoplasmic male sterility from oilseed rape (Brassica napus) to broccoli (B. oleracea) by protoplast fusion

✍ Stephen A. Yarrow; Laurie A. Burnett; Richard P. Wildeman; Roger J. Kemble 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 896 KB

Protoplast fusion was utilised to transfer Polima type cytoplasmic male sterility (CMS) from Brassica napus, canola cv. Polima Karat (Pol-Karat) to B. oleracea, broccoli, var. "Green Comet". Southern and RFLP analysis confirmed that four cybrids possessed nuclear genomes of broccoli with Polima mito