Production of monoclonal antibodies to study corrosion products of CO-CR biomaterials

✍ Scribed by Yang, Jun ;Merritt, Katharine

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 852 KB
• Volume: 31
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/(sici)1097-4636(199605)31:1<71::aid-jbm9>3.0.co;2-n

✦ Synopsis

Sensitivity to nickel, cobalt, and chromium is common among the general population. The identification of these sensitivities is generally by the detection of cell-mediated immunity. We have reported previously the use of an indirect enzymelinked immunosorbent assay method to quantitate metalspecific antibodies in patients with total joint replacements.

To study the haptenic potential of these metal ions, rabbit albumin-glutathione-metal complexes with chromium, cobalt, or nickel were injected into mice. The splenocytes from one mouse in each group which developed a strong antibody against GSH-metal complexes were isolated and fused with myeloma cells to produce monoclonal antibodies. Chromium, cobalt, and nickel antibodies had similar affinity and bound with the specific GSH-metal complex. There was very little cross-reactivity between these antibodies. An inhibition assay using these monoclonal antibodies was demonstrated to be a simple technique, suitable for quantitation of free metal in solution.