Production of laccase isoforms by Pleurotus pulmonarius in response to presence of phenolic and aromatic compounds

✍ Scribed by Cristina Giatti Marques De Souza; Giovana Kirst Tychanowicz; Daniela Farani De Souza; Rosane Marina Peralta

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 136 KB
• Volume: 44
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.200310365

✦ Synopsis

Abstract

The effect of several phenolic and aromatic monomers structurally‐related to lignin on production of laccase by the white rot fungus P. pulmonarius (Fr.) Quélet has been studied. In the absence of an inducer, laccase was maximally produced after depletion of carbon and nitrogen sources. Among 15 phenolic and aromatic compounds tested, ferulic acid and vanillin were the most efficient inducers, increasing the production of laccase activity up to 10 times. A mixture of ferulic acid and vanillin was more efficient to induce the production of laccase than the isolated phenolics. At least three laccase isoforms designated as __lcc__1, __lcc__2 and __lcc__3 were identified by eletrophoretic analysis of P. pulmonarius culture filtrates. The __lcc__1 and __lcc__2 isoforms were produced by non‐induced cultures, while __lcc__3 was found only in induced‐culture filtrates. (© 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)