Production of interspecific hybrids betweenGlycine maxandG. tomentellathrough embryo culture

✍ Scribed by Gyu Hwa Chung; Jin Hyeong Kim

• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 354 KB
• Volume: 48
• Category: Article
• ISSN: 0014-2336
• DOI: 10.1007/bf00037186

✦ Synopsis

Interspecific hybrids have been obtained in an incompatible cross between Glycine max and G . tomentella through the in vitro culture of hybrid embryos . The percentage of successful pod setting in the crosses averaged 12 .8% but there were marked differences depending on the soybean cultivar used as the female parent . Hybrid embryos at globular to heart stages were extracted from the embryo sac 15-25 days after pollination and cultured in vitro . Hybrid plants were successfully obtained by culturing the embryos on B5 medium supplemented with 0 .1 mg/l IBA followed by culture on B5 medium supplemented with 0 .1 mg/l IBA plus 0 .25 mg/l 2-iP . The F1 plants resembled the wild male parent in growth form, but had an intermediate leaf shape between that of the parents .