Production of identical twin and triplet mice by nuclear transplantation

✍ Scribed by Kono, T. ;Tsunoda, Y. ;Nakahara, T.

• Book ID: 102894167
• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 739 KB
• Volume: 257
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402570210

✦ Synopsis

Transplantation of a single nucleus from two- or four-cell embryos into one of the enucleated blastomeres of a two-cell embryo resulted in successful production of identical triplet and twin mice. The proportion of reconstituted embryos that developed in blastocysts was 71% (84/118) when four-cell embryos were used as donors of nuclei; 10 sets of quadruplet and nine sets each of triplet and twin blastocysts were obtained by this technique. After transfer to recipients, 30% (18/61) developed to term, and one set of identical triplet and four sets of identical twin mice were obtained. When two-cell embryos were used as donors of nuclei, 79 (95%) sets of twin embryos developed to blastocysts. Of 38 twin blastocysts transferred to recipients, 21 sets (55%) developed to term as identical twin mice. These results demonstrate that the enucleated two-cell embryo develops in vitro after transfer of a nucleus from a two- or four-cell embryo and the resultant blastocyst has high potential for development to term after transfer to a recipient.