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Production of human secreted alkaline phosphatase in suspension and immobilization cultures of tobacco NT1 cells

✍ Scribed by Naomi Shibasaki-Kitakawa; Takuya Miyamoto; Masaki Kubo; Nobuyuki Uozumi; Kinya Toriyama; Toshikuni Yonemoto; Michael L. Shuler

Book ID
113682776
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
55 KB
Volume
108
Category
Article
ISSN
1389-1723

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Influence of culture medium supplementat
Influence of culture medium supplementation of tobacco NT1 cell suspension cultures on the N-glycosylation of human secreted alkaline phosphatase
✍ Alejandro Becerra-Arteaga; Michael L. Shuler πŸ“‚ Article πŸ“… 2007 πŸ› John Wiley and Sons 🌐 English βš– 169 KB πŸ‘ 2 views

## Abstract We report for the first time that culture conditions, specifically culture medium supplementation with nucleotide‐sugar precursors, can alter significantly the N‐linked glycosylation of a recombinant protein in plant cell culture. Human secreted alkaline phosphatase produced in tobacco