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Production of human lysozyme-transgenic cloned porcine embryos by somatic nuclear transfer

✍ Scribed by Qiuyan Li; Hengxi Wei; Ying Guo; Yan Li; Rui Zhao; Yufang Ma; Zhengquan Yu; Bo Tang; Lei Zhang; Yunping Dai; Ning Li

Book ID
113855798
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
179 KB
Volume
19
Category
Article
ISSN
1002-0071

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In this study the in vitro development of porcine nuclear transfer (NT) embryos was investigated. Transgenic fetal fibroblast cells that were frozen after 5 days of serum starvation were injected immediately after thawing into enucleated metaphase II (MII) oocytes. Reconstructed embryos were activat