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Production of human epidermal growth factor by an ampicillin resistant recombinantEscherichia colistrain

✍ Scribed by V. B. Yadwad; S. Wilson; O. P. Ward

Publisher
Springer Netherlands
Year
1994
Tongue
English
Weight
330 KB
Volume
16
Category
Article
ISSN
0141-5492

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✦ Synopsis

The effect of medium composition and initial glucose concentration on production of hEGF by recombinant E. coli cells was investigated. Optimum hEGF production was observed in a yeast extract/acid hydrolysed casein/salts media containing an initial glucose concentration of 10 g.l 1. A maximum hEGF titer of 250 mg.l "1 was obtained in this medium after 32 h in laboratory fermenters with pH, temperature, agitation and aeration set at 6.8, 30"C, 500 rpm and 2 vvm, respectively.

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