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Production of high fructo-oligosaccharide syrup with two enzyme system of fructosyltransferase and glucose oxidase

โœ Scribed by Kyung Jung; Jeong Kim; Yeong Jeon; Jae Lee

Publisher
Springer Netherlands
Year
1993
Tongue
English
Weight
327 KB
Volume
15
Category
Article
ISSN
0141-5492

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โœฆ Synopsis

A novel two enzyme system of fructosyltransferase and glucose oxidase to enhance the content of the net fructo-oligosaccharide (FOS) fractions in the industrial production of FOS syrup from sucrose was devised.

The net FOS content in the commercial FOS syrup has been limited only to 55-60 % due to the accumulation of glucose which acts as a feedback inhibitor of the fructosyltransf erase.

By supplementing glucose oxidase to the conventional FOS reaction system, we could convert the glucose to gluconic acid readily separable from neutral sugars by simple ion exchange operation in the next step. The simultaneous removal of glucose was proved effective in proceeding the reaction by fructosyltransferase further by relieving the product inhibition caused by glucose.

By this way, we could raise the net FOS content as high as 90 %.

๐Ÿ“œ SIMILAR VOLUMES

The production of high-content fructo-ol
The production of high-content fructo-oligosaccharides from sucrose by the mixed-enzyme system of fructosyltransferase and glucose oxidase
โœ Won Yun Jong; Koo Song Seung ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› Springer Netherlands ๐ŸŒ English โš– 230 KB

A technique to produce high-content fructo-oligosaccharides by the mixed-enzyme system of fructosyltransferase and glucose oxidase was investigated. The mixed-enzyme reaction was carried out in a stirred tank reactor containing 40 %(w/v) sucrose with 10 unit of fructosyltransferase and 10 unit of gl