Production of heterologous proteins inBacillus subtilis: the effect of the joint between signal sequence and mature protein on yield

✍ Scribed by Harri Hemilä; Mervi Sibakov

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 417 KB
• Volume: 36
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00164699

✦ Synopsis

We have previously made a set of D N A constructs by fusing the mature part of Bacillus licheniformis a-amylase with the signal sequence of B. amyloliquefaciens a-amylase at various distances from the signal sequence cleavage site. We observed that the level of a-amylase production in B. subtilis depended strongly on the distance of the junction from the signal sequence cleavage site, with quite a sharp optimum distance. To test whether the effect is limited to the pair of a-amylase signal sequence and mature protein, we analysed the protein production in a set of constructs in which an Escherichia coli fl-lactamase was similarly joined at different distances from the a-amylase signal sequence. Also in this case the distance seemed to be an important factor in affecting the level of production in B. subtilis. The observed effect might depend on the modulation of pre-protein folding, which in turn could affect the secretion level.