Production of heparin related glycosaminoglycans by an established mammalian cell line

✍ Scribed by Paul M. Kraemer

• Book ID: 102878802
• Publisher: John Wiley and Sons
• Year: 1968
• Tongue: English
• Weight: 906 KB
• Volume: 71
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040710202

✦ Synopsis

A sulfated glycosaminoglycan has been isolated from the acid-soluble fraction of an established line of Chinese hamster fibroblasts grown in suspension culture. This material has a molecular weight between 5000 and 10,000, contains equimolar amounts of hexosamine and uronic acid (orcinol method), and about 0.6 sulfate groups per hexosamine residue. About 80% of the sulfate groups are N-sulfates on the basis of lability of the sulfate and the formation of equivalent numbers of free amino groups upon mild acid hydrolysis. The material is completely resistant to testicular hyaluronidase but is degraded to reducing monosaccharides and small oligosaccharides upon treatment with lyophilized cells of Flavobacterium heparinum that were grown on heparin. It is thought, therefore, to be related to the known N-sulfated glycosaminoglycans heparin and heparitin sulfate.