Production of hemo- and immunoregulatory cytokines by erythroblast antigen+and glycophorin A+cells from human bone marrow
✍ Scribed by Sergey V Sennikov; Tatyana V Injelevskaya; Sergey V Krysov; Alexandr N Silkov; Igor B Kovinev; Natalya J Dyachkova; Anton N Zenkov; Mary I Loseva; Vladimir A Kozlov
- Publisher
- BioMed Central
- Year
- 2004
- Tongue
- English
- Weight
- 343 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1471-2121
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✦ Synopsis
Background
Erythroid nuclear cells (ENC) of the bone marrow (BM) have not previously been considered as important producers of wide spectrum of haemo- and immunoregulatory cytokines. The aim of the current work was to confirm the production of the main hemo- and immunoregulatory cytokines in human ENC from BM.
Results
We used native human BM ENC in our experiments. We for the first time have shown, that the unstimulated erythroblasts (Gl A^+^ or AG-EB^+^) produced a wide spectrum of immunoregulatory cytokines. Human BM ENC produce cytokines such as interleukn (IL)-1β, IL-2, IL-4, IL-6, interferon (IFN)-γ, transforming growth factor (TGF)-β1, tumor necrosis factor (TNF)-α and IL-10. They can be sub-divided into glycophorin A positive (Gl A^+^) and erythroblast antigen positive (AG-EB^+^) cells. To study potential differences in cytokine expression between these subsets, ENC were isolated and purified using specific antibodies to Gl A and AG-EB and the separated cells were cultivated for 24 hours. The cytokine contents of the supernatant were measured by electrochemiluminescence immunoassay. Quantitative differences in TGF-β1 and TNF-α production were found between Gl A^+^ and AG-EB^+^ BM ENC. Furthermore, in vitro addition of erythropoietin (EPO) reduced IFN-γ and IL-2 production specifically by the AG-EB^+^ ENC. Thus, Gl A^+^ and AG-EB^+^ ENC produce IL-1β, IL-2, IL-4, IL-6, IFN-γ, TGF-β1 and TNF-α. Gl A^+^ ENC also produce IL-10.
Conclusion
Cytokine production by erythroid nuclear cells suggests that these cells might be involved in regulating the proliferation and differentiation of hematopoietic and immunocompetent cells in human BM.