Production of GST–SOD fusion protein by recombinant E coli XL1 Blue

Connection between gene dosage and prote

Connection between gene dosage and protein stability revealed by a high-yield production of recombinant proteins in an E. coli LexA1(Ind−) background

✍ Manel G. Medina; Xavier Carbonell; Antonio Villaverde 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 174 KB

## Abstract Bacterial production of a plasmid‐encoded bacteriophage P22 tailspike protein shows different yield and impact on cell viability in RecA^+^ LexA^+^, RecA^−^ LexA^+^ and RecA^+^ LexA1(Ind^−^) backgrounds. In a LexA1(Ind^−^) context, we have observed lesser toxicity and higher productivit