Production of granulocyte-macrophage colony stimulating factor (GM-CSF) by high cell density fermentation of secretory recombination Escherichia Coli
โ Scribed by Xue-Wu Zhang; Tao Sun; Xin Liu; De-Xiang Gu; Zhan-Qui Tang
- Book ID
- 114080952
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 113 KB
- Volume
- 34
- Category
- Article
- ISSN
- 1873-3298
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
## Abstract Spleen cells were collected from normal mice and cultured in a medium containing 20% calf serum. Addition of lipopolysaccharide (LPS) in the culture significantly increased the production of granulocyteโmacrophage colonyโstimulating factor (GMโCSF), and a maximum induction was attained
## Abstract Seven days after subcutaneous injection of 2% carrageenin solution in mice, the induced inflammatory tissue was isolated and treated with 0.1% trypsin. When the dispersed cells were incubated in a nutrient medium containing 5โโ20% calf serum, the cells grew adhering to the cultureโdish