Production of G-protein-coupled receptors in yeast

✍ Scribed by Helmut Reiländer; H Markus Weiβ

• Publisher: Elsevier Science
• Year: 1998
• Tongue: English
• Weight: 684 KB
• Volume: 9
• Category: Article
• ISSN: 0958-1669
• DOI: 10.1016/s0958-1669(98)80038-4

✦ Synopsis

Yeasts combine the advantages of fast and easy handling with the potential to perform eukaryotic post-translational modifications and are for this reason interesting hosts for heterologous production of G-protein-coupled receptors. The possibility to connect foreign receptors to a yeast internal MAP kinase pathway was used to establish yeast-based systems for high-throughput screening of compound libraries. In addition, yeasts have the potential for high level production of G-protein-coupled receptors. In this field, non-Saccharomyces yeasts seems to be interesting alternatives to S. cerevisiae, as well as to systems based on higher eukaryotic cells.