Production of fertile transgenic wheat plants via tissue electroporation
β Scribed by Alexander P Sorokin; Xia-Yi Ke; Dong-Fang Chen; Malcolm C Elliott
- Book ID
- 117382269
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 628 KB
- Volume
- 156
- Category
- Article
- ISSN
- 0168-9452
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Maize Type II callus tissue was used as the plant material for genetic transformation via electroporation. Plasmid DNA containing a selectable marker gene (either neomycin phosphotransferase (npt-II) or phosphinothricin acetyl transferase (bar)), and a screenable marker gene (gus A) was incubated wi
We describe an efficient procedure for genetic transformation of commercial sugarcane varieties POJ 2878 and Ja 60-5. The transformation protocol is based on electroporation of a plasmid conferring GUS activity into cell clusters isolated from embryogenic calli. Six to eight weeks after electroporat