Production of extracellular polysaccharides by a Rhizobium species from the root nodules of Tephrosia purpurea Pers

✍ Scribed by P. S. De; P. S. Basu

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 402 KB
• Volume: 16
• Category: Article
• ISSN: 0138-4988
• DOI: 10.1002/abio.370160210

✦ Synopsis

The Rhizobium sp., isolated from the root nodules of the leguminous climbing herb, Tephrosia Purpurea Pers., produced high amounts of extracellular polysaccharides (EPS) in yeast extract mannitol medium. Growth and EPS production started simultaneously, but the production reached its maximum in the stationary growth phase of the bacteria. Attempts were made to optimize the cultural requirements for growth and maximum EPS production. The EPS production was increased by 72.5% over the control when the medium was supplemented with mannitol (2%). thiamine hydrochloride (5 pglml) and KN@ (0.1%). The EPS contained glucose, galactose and mannose monomers. The possible role of the rhizobial EPS was discussed.