Production of cell wall-degrading enzymes by three isolates of Aspergillus niger under different cultivation conditions
✍ Scribed by A. Mehta; S. Chopra; Dr. P. Mehta
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 387 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
Three strongly pathogenic isolates of Aspergillus niger were capable of producing pectolytic, cellulolytic and proteolytic enzyme activities in various culture media, extracellularly, indicating the positive correlation between in vitro enzyme production and virulence of the pathogens.
Cell wall-degrading enzymes play an important role during pathogenesis (ALBERSHEIM et . Production of individual enzymes by the pathogens in a culture medium and the amount of activity in a crude enzyme extract are influenced by components of the culture media . Certain substances present in the medium induce the secretion of enzymes , while other substances inhibit the secretion of enzymes . Many workers (HUSAIN and RICH 1958, VANCE et al. 1980) observed the production of cell wall-degrading enzymes in different culture media. Both, natural (MEHTA and MEHTA 1985, MISHRA 1978) and synthetic media (MEHTA and MEHTA 1985, HUSAIN and have been widely used for the production of pectolytic and cellulolytic enzymes. REDDY et al. (1980) studied the effect of five synthetic media on the production of protease enzyme by Aspergillus niger. Production of cell wall-degrading enzymes in the diseased fruits infected with these isolates and also in culture provided the reason for studying the characteristics of these cell wall-degrading enzymes in detail. Therefore, it was thought desirable to determine the optimum cultural conditions for the production of these enzymes in vitro.